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Abstract Detail

Recent Topics Posters

Moore, Brianna [1], Carlson, Craig [2], Gouker, Fred [2], Straub, Shannon C.K. [1], Smart, Lawrence [2].

Genetics of sex determination in the dioecious shrub willow, Salix purpurea.

Salix purpurea (Salicaceae) is one of small percentage of angiosperms that are dioecious, and a good model system for the study of sex chromosome evolution. A sex determination region for this species has been identified on chromosome 15. The aim of this study was to characterize the genetic differences that determine sex. Towards this aim, PCR primers were designed to amplify two genes, mediator of RNA polymerase II and TCP24, which occur within the previously identified sex determination region. DNA sequences obtained from male and female individuals were aligned to identify possible polymorphisms that segregate with sex. Analysis showed results to be consistent with a ZW system, where males are homozygous and females are heterozygous. Restriction sites were identified and digestions were completed to develop CAPS polymorphisms that can be used for identification of an individual’s sex early in development. Further research can be conducted with the goal of cloning genes to sequence the female-specific allele, which may can be hard to identify due to length differences between the two alleles in these heterozygous individuals.

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1 - Hobart and William Smith Colleges, Department of Biology, 300 Pulteney Street, Geneva, NY, 14456, USA
2 - Cornell University, Environmental & Forest Biology, NYS Ag Experiment Station, 630 West North St., Geneva, NY, 14456, USA

Salix purpurea
sex determination region

Presentation Type: Recent Topics Poster
Session: P, Recent Topics Posters
Location: Exhibit Hall/Omni Hotel
Date: Monday, June 26th, 2017
Time: 5:30 PM
Number: PRT016
Abstract ID:765
Candidate for Awards:None

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