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Abstract Detail

Recent Topics Posters

Wanke, Stefan [1], Granados Mendoza, Carolina [2], Müller, Sebastian [3], Paizanni Guillén, Anna [4], Neinhuis, Christoph [3], Lemmon, Alan R. [5], Moriarty Lemmon, Emily [6], Samain, Marie-Stéphanie [7].

Recalcitrant deep and shallow nodes in Aristolochia (Aristolochiaceae) illuminated using anchored hybrid enrichment.

Recalcitrant relationships are characterized by very short internodes that can be found among shallow and deep phylogenetic scales all over the tree of life. Adding large amounts of presumably informative sequences, while decreasing systematic error, has been suggested as a possible approach to increase phylogenetic resolution. The development of enrichment strategies, coupled with next generation sequencing, resulted in a cost-effective way to facilitate the reconstruction of recalcitrant relationships. By applying the anchored hybrid enrichment (AHE) genome partitioning strategy to Aristolochia using an universal angiosperm probe set, we obtained 231–233 out of 517 single or low copy nuclear loci originally contained in the enrichment kit, resulting in a total alignment length of 154,756 bp to 160,150 bp. Since Aristolochia (Piperales; magnoliids) is distantly related to any angiosperm species whose genome has been used for the plant AHE probe design (Amborella trichopoda being the closest), it serves as a proof of universality for this probe set. Aristolochia comprises approximately 500 species grouped in several clades (OTUs), whose relationships to each other are partially unknown. Previous phylogenetic studies have shown that these lineages branched deep in time and in quick succession, seen as short-deep internodes. Short-shallow internodes are also characteristic of some Aristolochia lineages such as Aristolochia subsection Pentandrae, a clade of presumably recent diversification. This subsection is here included to test the performance of AHE at species level. Filtering and subsampling loci using the phylogenetic informativeness method resolves several recalcitrant phylogenetic relationships within Aristolochia. By assuming different ploidy levels during bioinformatics processing of raw data, first hints are obtained that polyploidization contributed to the evolution of Aristolochia. Phylogenetic results are discussed in the light of current systematics and morphology.

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1 - TU Dresden, Dept. Of Biology, Institut Of Botany, Zellescher Weg 20b, Dresden, N/A, 01062, Germany
2 - CONACyT IPICyT, Division de Biologia Molecular, Camino a la Presa de San Jose 2055, Lomas 4a. seccion, San Luis Potosi, San Luis Potosi, 78216, Mexico
3 - Technische Universität Dresden, Institut für Botanik, Zellescher Weg 20b, Dresden, 01062, Germany
4 - Universidad Michoacana de San Nicolás de Hidalgo, Morelia, Michoacan, 58066, Mexico
5 - Florida State University, Department of Scientific Computing, Dirac Science Library, Tallahassee, FL, 32306-4102, USA
6 - Florida State University, Department of Biological Science, 319 Stadium Drive, Tallahassee, FL, 32306-4295, USA
7 - Instituto de Ecología, A.C., Centro Regional del Bajío, Pátzcuaro, Michoacán, 61600, Mexico

Enrichment strategy
next generation sequencing
Short internodes
Recent diversification
Universal nuclear probe set.

Presentation Type: Recent Topics Poster
Session: P, Recent Topics Posters
Location: Exhibit Hall/Omni Hotel
Date: Monday, June 26th, 2017
Time: 5:30 PM
Number: PRT002
Abstract ID:728
Candidate for Awards:None

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