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Abstract Detail


NOYES, RICHARD DAVID [1], KLING, Brittany [1].

Whole chloroplast sequencing for Asteraceae tribe Astereae; methodology and phylogenetic ramifications.

Tribe Astereae, (Asteraceae), includes about 170 genera and 2,800 species making it the second largest tribe in the family. The tribe is of worldwide distribution and woefully understudied phylogenetically. Here we conducted complete chloroplast genome sequencing for 10 diverse species including Southern Hemisphere genera Baccharis and Felicia, and North American genera Boltonia, Bradburia, Doellingeria, Erigeron (2 sp.), Euthamia, Felicia, Solidago, and Symphyotrichum. We used a high-salt enrichment procedure and MiSeq (2 x 300bp) short read Illumina sequencing. Results completely resolve the 10 genomes and show that their sequences are generally consistent in terms of overall size, gene content, and order with other Asteraceae. Most of the size differences among samples is due to expansion or contraction of intergenic regions. Phylogenetic analyses rooted using sequences for sister-tribe Anthemidae resolve Felicia as basal within Astereae, as expected. However, conspicuous conflicts with nrDNA data are supported including the failure to resolve the eight North American genera as monophyletic.

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1 - University Of Central Arkansas, Department Of Biology, Lewis Science Center 180, Conway, AR, 72035, USA

plastid genome

Presentation Type: Oral Paper
Session: 33, Phylogenomics II
Location: Fort Worth Ballroom 4/Omni Hotel
Date: Wednesday, June 28th, 2017
Time: 9:15 AM
Number: 33006
Abstract ID:528
Candidate for Awards:None

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