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Abstract Detail

Molecular Ecology

Garza, Elyssa [1], Hawkins, Angela [1], Pepper, Alan [1].

Genome comparison of two Caulanthus varieties to identify possible loci contributing to serpentine tolerance.

A wild relative of Arabidopsis, Caulanthus amplexicaulis var. barbarae (Cab) is endemic to harsh serpentine soils; characterized by low calcium-to-magnesium ratios, increased heavy metal presence, minimal nutrients, and low water retention. Its sister variety, Caulanthus amplexicaulis var. amplexicaulis (Caa), inhabits adequate nutrient granite soils. The distinct contrast between the natural habitats of these two plant varieties allows exploration into plant selective adaptations to serpentine endemism. We have assembled, analyzed, and annotated the Cab and Caa genomes. The estimated genome size for C. amplexicaulis is 372 Mb; with 14 chromosomes. Along with resequencing data from a set of recombinant inbred lines, we are able to identify valuable marker and recombination data. Using a cluster of genomic prediction software, we have identified variants such as SNVs, indels, and structural variation to locate genes that possibly affect varying tolerance to multiple soil types. Information collected from currently available genomic and QTL programs-variants detected, recombination breakpoints, chromosome orientations, and annotations-have allowed the construction of a draft genetic map with high-density marker profiles and the potential to identify loci involved in serpentine tolerance.

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1 - Texas A & M University, Department of Biology, 100 Butler Hall, 3258 TAMU, College Station, TX, 77843, USA

edaphic endemism
whole genome resequencing.

Presentation Type: Poster
Session: P, Molecular Ecology
Location: Exhibit Hall/Omni Hotel
Date: Monday, June 26th, 2017
Time: 5:30 PM This poster will be presented at 6:15 pm. The Poster Session runs from 5:30 pm to 7:00 pm. Posters with odd poster numbers are presented at 5:30 pm, and posters with even poster numbers are presented at 6:15 pm.
Number: PME002
Abstract ID:502
Candidate for Awards:None

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