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Abstract Detail

Population Genetics/Genomics

Thapa, Ramhari [1], Bayer, Randall [2], Mandel, Jennifer R. [3].

Development of Microsatellite Markers in Antennaria (Asteraceae) Using Genomic Data.

Antennaria is a peculiar genus of the tribe Gnaphalieae (Asteraceae) with features such as polyploidy, sexual dimorphism, gametophytic apomixis etc. The genus mainly has a Holarctic distribution unlike most of the other members of the Tribe Gnaphalieae which are prevalent in South America, Southern Africa and Australia. It is a moderate size genus of about 40 recognized species divided into basal Leontipes and advanced Catipes groups, with the members of Catipes groups giving rise to at least five polyploid agamic complexes by intense hybridization and backcrossing. Antennaria rosea is one of the most morphologically diverse polyploidy agamic complexes sharing similar morphology with eight putative Antennaria parents: A.aromatica, A. corymbosa, A. microphylla, A. marginata, A. pulchella, A. rosulata, A. racemosa, and A. umbrinella. In this study, genomic data previously obtained by sequencing more than 1000 nuclear Conserved Ortholog Set (COS) loci for the reconstruction of phylogenetic relationship among recognized Antennaria species and also sequence data produced by enrichment of Antennaria SSR loci were used to develop SSR markers to investigate polyploid origin and patterns of clonal diversity in the A. rosea species. Sequence data from COS loci and SSR enriched loci for the eight putative Antennaria parents were first aligned using MAFFT in the Geneious software platform and the SSR search tool, PHOBOS, was used to locate possible repeat regions, which were then selected based on differences in the number of repeats seen in the alignment. Similarly, SSR loci were also searched in the partially assembled chloroplast data from the off-target reads of COS loci. Sixteen primer pairs were developed, seven pairs from nuclear COS loci, four pairs from SSR enriched loci, and five pairs from chloroplast assembled data, and tested for amplification. All the primer pairs, except one from the nuclear COS loci, amplified for the putative A. rosea parents as well as a few tested A. rosea populations.

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1 - University of Memphis, 3700 Walker Avenue, Memphis, TN, 38152, United States
2 - University of Memphis, 3700 Walker Avenue , Memphis, TN, 38152, United States
3 - University Of Memphis, 3744 Walker Ave, Memphis, TN, 38152, USA

Conserved Orthologos Set

Presentation Type: Poster
Session: P, Population Genetics/Genomics
Location: Exhibit Hall/Omni Hotel
Date: Monday, June 26th, 2017
Time: 5:30 PM This poster will be presented at 5:30 pm. The Poster Session runs from 5:30 pm to 7:00 pm. Posters with odd poster numbers are presented at 5:30 pm, and posters with even poster numbers are presented at 6:15 pm.
Number: PPG005
Abstract ID:447
Candidate for Awards:None

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