Abstract Detail

Evolutionary Developmental Biology (Evo-Devo)

Tong, Jingjing [1], Berger, Brent [2], Knox, Eric [3], Morden, C. W. [4], Cellinese, Nico [5], Pender, Richard [6], Howarth, Dianella [2].

Duplication and expression of CYCLOIDEA-like genes in Campanulaceae.

Shifts in flower symmetry between radial symmetry and bilateral symmetry have evolved multiple times independently in angiosperms and have played a major role in speciation and adaptation. Evidence from studies of core eudicots indicate that CYCLOIDEA-like (CYC) genes, which belong to the class II TCP gene family, specify dorsal identify in bilateral symmetrical flowers. Three core eudicot clades of CYC-like genes have been identified: CYC1, CYC2 and CYC3. This study focuses on these gene clades in Campanulaceae, which includes five subfamilies, with one, Campanuloideae, having radial symmetrical flowers. All other subfamilies, including Lobelioideae, Cyphioideae, and Cyphocarpoidea, have bilaterally symmetrical flowers. At present, we have sequenced CamCYC-like genes from Campanuloideae, Lobeliodeae, and Cyphioideae. Our data indicate that CamCYC2 genes have duplicated in Lobeliodeae, correlated with a shift to bilateral symmetry in Campanulaceae. Current sampling from CamCYC3 genes indicates that they have duplicated at least in the bilaterally symmetrical Cyphioideae. We aim with further work to utilize real time RT-PCR to examine expression patterns of different paralogs of CamCYC-like genes in Campanuloideae (radial flowers) and Lobeliodeae (bilateral flowers). Additionally, we aim to examine the localization of CamCYC-like genes in radial and bilateral flowers in Campanulaceae via in situ hybridization.

1 - St. John's University, 8000 Utopia Pkwy, Jamaica, NY, 11439, USA
2 - St. John's University, 8000 Utopia Pkwy, Jamaica, NY, 11439, United States
3 - Indiana University, Department of Biology, Bloomington, IN, 47405, USA
4 - University Of Hawaii, Department Of Botany, 3190 Maile Way, HONOLULU, HI, 96822-2279, USA
5 - University of Florida, Florida Museum of Natural History, 1659 Museum Road, Gainesville, Florida, 32611, United States
6 - New Zealand Department of Conservation, Wellington, NZ

Keywords:
CYCLOIDEA
Floral symmetry
gene expression
Campanulaceae
gene duplication.

Presentation Type: Oral Paper
Session: 34, Evo-Devo
Location: Sundance 2/Omni Hotel
Date: Wednesday, June 28th, 2017
Time: 10:30 AM
Number: 34010
Abstract ID:429
Candidate for Awards:Katherine Esau Award