| Abstract Detail
Classical Genetics Gendron, Jake [1], Pabuayon, Isaiah [2], Kitazumi, Ai [2], Karampudi, Bhushan [2], Pal Kaur, Pushpinder [2], Cushman, Kevin [2], Singh, R.K. [3], Gregorio, Glenn [3], de los Reyes, Benildo [2]. Understanding the molecular basis of transgressive phenotypes in rice: A case study on novel dehydration stress tolerance mechanisms in recombinant inbred lines derived from dehydration-sensitive parents. In order to address the ‘ten billion people question’, the mechanisms behind genetic network rewiring and how they contribute to novel stress tolerance phenotypes in transgressive segregants produced by conventional breeding must be elucidated. Such an effort represents the marriage of the classic paradigms of plant breeding and the modern paradigms of genome biology, towards climate-resilient rice varieties for the 21st century. We reexamined the recombinant inbred line (RIL) population derived from IR29 (salinity-sensitive) x Pokkali (salinity-tolerant) within the context of responses to dehydration (i.e., 10% PEG8000 in hydroponics). Capitalizing on the quantitative physiological variation for salinity stress that we revealed in this population, of which some parameters appeared to be relevant also to dehydration, we pose the question of whether transgressive dehydration tolerance might exist in this population although neither of the parents was drought tolerant. To this end, using this simple experimental system, we are testing the hypothesis that physiological factors responsible for salinity tolerance in Pokkali may be recombined with various cryptic but complementary or enhancing attributes of IR29, to reconfigure the dehydration response mechanisms. Our results suggest that recombination between IR29 and Pokkali led to novel combinations of cryptic physiological attributes from each parent, creating positive effects in certain RILs. Transgressive phenotypes for salinity and dehydration segregated independently, implying distinct network configurations in the transgressive RILs for each trait. We present the results of our initial efforts to dissect the rewired regulatory and physiological networks among transgressive RILs at the levels of mRNA and miRNA transcriptomes.
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1 - Texas Tech University, Plant and Soil Sciences, Box 4212, Lubbock, Texas, 79409, USA
2 - Texas Tech University, Box 4212, Lubbock, Texas, 79409, United States
3 - IRRI, DAPO Box 7777, Metro Manila 1301, Philippines
Keywords:
none specified
Presentation Type: Poster
Session: P, Classical Genetics
Location: Exhibit Hall/Omni Hotel
Date: Monday, June 26th, 2017
Time: 5:30 PM This poster will be presented at 6:15 pm. The Poster Session runs from 5:30 pm to 7:00 pm. Posters with odd poster numbers are presented at 5:30 pm, and posters with even poster numbers are presented at 6:15 pm.
Number: PCG002
Abstract ID:418
Candidate for Awards:Genetics Section Poster Award |
