| Abstract Detail
Pteridology Kuo, Li-Yaung [1], Tang, Te-Yen [2], Chiou, Wen-Liang [3], Li, Fay-Wei [1], Huang, Yao-Moan [2], Wang, Chun-Neng [4]. Organelle genome inheritances in Deparia ferns (Athyriaceae, Aspleniineae, Polypodiales). Organelle genome inheritances of land plants are very diverse in that they can be inherited maternally but also paternally or biparentally. Despite much being known in seed plants (> 83% genera of angiosperm and > 12% genera of gymnosperms), organelle genome inheritances have only been investigated in fewer than 2% of fern genera. Besides, inheritance of mitochondrial genome has been investigated only in one fern genus. This study aims on broadening knowledge of organelle genome inheritances in ferns by understanding this from an additional and uninvestigated lineage. We examined organelle genome inheritances of both plastid and mitochondrion in Deparia ferns (Athyriaceae, Aspleniineae, Polypodiales) using a new and efficient approach to investigate organelle DNA transmission in infra-specific crosses and inter-specific hybrids. In practice, we first cultured a spore mixture of two individuals of the diploid D. lancea, and then sequenced their organelle genomes in order to find pDNA (plastid DNA) and mtDNA (mitochondrial DNA) SNP (Single Nucleotide Polymorphism) as genetic markers tracing organelle DNA transmission. To confirm the maternal parent of the newly generated sporophytes, we genotyped each of their attached gametophytes with the aid of tissue-direct PCR; and the identity of their paternal parents was verified by genotyping the nuclear locus, IBR3. Next, to examine whether or not both organelle genomes have a same inheritance way in Deparia inter-species hybrids, we sequenced both pDNA and mtDNA regions of nine D. ×tomitaroana individuals, and performed phylogenies using these regions in order to identify the origins of organelle DNA in these hybrids. In sum, the evidences from our experiments and analyses support that both organelle genomes in Deparia are uniparentally and maternally inherited. Most importantly, our study is the first one reporting mtDNA inheritance in eupolypod ferns, and the second one among all ferns.
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1 - Boyce Thompson Institute, USA
2 - Taiwan Forestry Research Institute, Taiwan
3 - Dr. Cecilia Koo Botanic Conservation Center, Taiwan
4 - Department of Life Science, National Taiwan University, Taiwan
Keywords:
none specified
Presentation Type: Oral Paper
Session: 6, Pteridological Section/AFS
Location: Sundance 1/Omni Hotel
Date: Monday, June 26th, 2017
Time: 10:15 AM
Number: 6004
Abstract ID:377
Candidate for Awards:None |
