Abstract Detail

Comparative Genomics/Transcriptomics

Andersen, Ethan [1], Nepal, Madhav P. [2].

Evolution of the NB-ARC Protein Domain as a Major Signaling Component of the Plant Defense Response.

Most of the plant disease resistance proteins are NB-LRR proteins, which are part of the NB-ARC domain (Nucleotide binding, apoptotic protease-activating factor-1, R proteins and CED-4 (Caenorhabditis elegans death-4 protein) in proteins across diverse segments of the tree of life. Animals, plants, fungi, and bacteria utilize proteins with NB-ARCs in signaling pathways for various cellular responses. Such proteins are highly diversified among plants, playing a major role in the detection of pathogen effectors in order to trigger a defense response. In this study, we aim to elucidate details of NB-ARC evolution across major plant lineages. The NB-ARC sequences were found in Ananas comosus, Amborella trichopoda, Physomitrella patens, Selaginella moellendorffii, Spirodela polyrhiza, Zostera marina, members of the genus Anabaena (cyanobacteria), and various other bacterial species. Highly conserved motifs within the NB-ARC, especially the P-loop and Kinase-2 motifs, are very similar even among distantly related species, indicating nonsynonymous substitutions in the amino acid sequence are selected against across diverse groups of organisms. The Chlamydomonas reinhardtii genome contained no NB-ARC protein-encoding sequences while there are dozens of NB-ARC proteins in the A. trichopoda, which likely are ancestral to the vast numbers of NB-ARC protein-encoding genes in grass genomes. Few NB-ARC-encoding sequences were found in cyanobacteria. We will discuss data revealing intriguing patterns of NB-ARC evolved for defense surveillance across plant lineages.

Related Links:
Nepal Lab @SDSU

1 - South Dakota State University , Biology and Microbiology , McFadden Biostress Laboratory, Brookings , SD, 57007
2 - South Dakota State University, Biology and Microbiology, McFadden Biostress Laboratory, Brookings, SD, 57007, USA

Keywords:
Evolution of plant immunity
plant defense surveillance
disease resistant genes
NBS-LRR proteins.

Presentation Type: Poster
Session: P, Comparative Genomics and Transcriptomics
Location: Exhibit Hall/Omni Hotel
Date: Monday, June 26th, 2017
Time: 5:30 PM This poster will be presented at 6:15 pm. The Poster Session runs from 5:30 pm to 7:00 pm. Posters with odd poster numbers are presented at 5:30 pm, and posters with even poster numbers are presented at 6:15 pm.
Number: PGT006
Abstract ID:311
Candidate for Awards:Genetics Section Poster Award