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Abstract Detail

Comparative Genomics/Transcriptomics

Andersen, Ethan [1], Nepal, Madhav P. [1], Ali, Shaukat [2], Yen, Yang [1], Neupane, Surendra [1].

Genome-wide identification of disease resistant genes in wheat (Triticum aestivum L.; Poaceae) .

Growing wheat cultivars resistant to yield-reducing pathogens provides a relatively cheap and environmentally friendly method for combating wheat diseases. Recently available complete genome sequences of wheat (Triticum aestivum) and its progenitors (T. urartu and Aegilops tauschii) allowed us to study evolution and diversity of disease resistant genes (R genes) — the Coiled-coil, Nuceotide-binding-site, and Leucine-rich repeat (CNL) genes. In this study, we identified and analyzed the CNL gene orthologs in the genomes of T. aestivum, T. urartu, and A. tauschii. We identified 609, 318, and 392 CNL genes in T. aestivum, T. urartu, and A. tauschii, respectively. Consistent with our previous studies, we found that all three monocot genomes lack Clade D which is present in the genomes of dicot species. Selection pressure analyses showed that CNL genes experienced purifying selection and exhibited evolutionary patterns similar to other grass species. Implementation of a duplication-loss model shows numerous instances where one or two of the three species experienced gene duplication or species-specific loss of their orthologs. Homology to many previously characterized resistance genes such as RPM1, RPP13, RPS2, Lr21, Yr10, MLA etc was observed, providing a basis for functional characterization. Future studies should aim at functional characterization of these genes and understand their roles in signaling pathways.

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Related Links:
Nepal Lab@South Dakota State

1 - South Dakota State University, Biology and Microbiology, McFadden Biostress Laboratory, Brookings, SD, 57007, USA
2 - South Dakota State University, Plant Science , Brookings, SD, 57007, United States

CNL disease resistant genes
purifying selection
gene duplication
gene diversification and maintenance .

Presentation Type: Poster
Session: P, Comparative Genomics and Transcriptomics
Location: Exhibit Hall/Omni Hotel
Date: Monday, June 26th, 2017
Time: 5:30 PM This poster will be presented at 5:30 pm. The Poster Session runs from 5:30 pm to 7:00 pm. Posters with odd poster numbers are presented at 5:30 pm, and posters with even poster numbers are presented at 6:15 pm.
Number: PGT005
Abstract ID:275
Candidate for Awards:Genetics Section Poster Award

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