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Abstract Detail

Conservation Biology

Cohen, Jim [1].

The conservation biology of Oreocarya crassipes (Boraginaceae), the Terlingua Creek Cat’s-Eye.

Oreocarya crassipes (Boraginaceae) is an endangered species endemic to Brewster Co., Texas in the area just north of Big Bend National Park. O. crassipes is a heterostylous species known from a small number of populations along the Fizzle Flat Lentil, and the species has edaphic specialization within the area. While aspects of the ecology of the species are known, the breeding system, genetic and genomic diversity, and population structure have not been examined. Four populations of O. crassipes were visited during 2014 and 2015, and flowers and leaves were collected. Using measurements of anther and stigma height from mature flowers, the extent of heterostyly was quantified between morphs and among populations. These measurements demonstrated that heterostyly is well established in the species. Additionally, cell length differences were investigated between the two morphs, and long-style morph corolla and stylar cells are longer than those of the short-style morph. Along with floral measurements, single nucleotide polymorphisms (SNPs) were identified using tunable genotyping-by-sequencing (tGBS) and microsatellite loci were sequenced. Genetic diversity, population structure, loci under selection, genetic bottlenecks, and patterns of migration and demographic history were examined using various methodologies. In general, FST values of the populations range from 0.5 to 0.1, and FST values are greater for microsatellite loci than SNP data. These FST values suggest little differentiation across the sampled populations. Additionally, FIS values for the populations range from slightly greater than 0 to 0.15, providing evidence that inbreeding occurs within the sampled populations. As the number of SNPs increases, along with a greater amount of missing data, FST and FIS values increase as well. Results of population structure analyses suggest that the sampled individuals comprise two genetically structured groups, although population structure is also observed should the sampled individuals be divided among three groups. Analyses of migration and demographic history provide evidence of migration among the four populations, although some populations have exchanged more migrants than others. Indeed, a unidirectional model of migration from the more southern population to the more northern populations is to be preferred over other models of migration for the four populations.

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1 - Kettering University, Applied Biology, 1700 University Ave., Flint, MI, 48504, USA


Presentation Type: Oral Paper
Session: 36, Conservation Biology
Location: Sundance 1/Omni Hotel
Date: Wednesday, June 28th, 2017
Time: 11:45 AM
Number: 36007
Abstract ID:267
Candidate for Awards:None

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