Abstract Detail

Comparative Genomics/Transcriptomics

Centeno, Chris [1], Jessup, Russell [2], Hatch, Stephan [3], Stelly, David [4].

Development of PCR-based Transposable Element Assays for Verification of Pearl Millet-Napiergrass Hybrids.

Texas Pearl Millet-Napiergrass (PMN) (Pennisetum glaucum [L.] R. Br (AA genome; 2n=2x=14) x Pennisetum purpurem Schumach. (A’A’BB genome; 2n=4x=28)) is a sterile triploid hybrid (2n = 3x = 21 chromosomes; AA’B genome). Constructing molecular verification methods using marker assays is crucial for distinguishing PMN hybrids from their parents and other hybrids, as morphological traits overlap significantly, nearly equivalent nuclear genome sizes between the parents prevents utilization of flow cytometry, and cytological chromosome determination is laborious. The reciprocal hybridization is also fertile, with P. purpurem x P. glaucum referred to as Kinggrass (KG). PMN produces more biomass than the parents but less than KG. Both KG and PMN have larger seed than parents and are morphologically similar to Naipergrass.Development of rapid marker assays will focus on variation of transposable elements within the parents then compared to PMN. Distinguishing hybrid species by the use of general taxonomic characteristics is very time consuming and meticulous but will be a secondary method of verification for generating uniform hybrids from the parents. Hybrid inflorescences will be examined for morphological differences and to verify seed sterility.

1 - Texas A & M University , Ecosystem Science and Management , 2138 tamu, College Station, Texas, 77843, usa
2 - Texas A & M University, Soil Crop Science , 431C Heep Center, College Station, Texas, 77843, United States
3 - Texas A & M University, Rm. 131B University Services Bldg. tamu, College Station, Texas, 77843, United States
4 - Texas A & M University, Beasley Laboratory Bldg. 965, Agronomy Rd. tamu, College Station, Texas, 77843, United States

Keywords:
Pennisetum glaucum
Pennisetum purpureum
Transposable Elements
qPCR
Comparative Genomics
Pearl Millet-Napiergrass Hybrids 
Pearl Millet
Napiergrass
Marker Survey
Vegetation survey methods comparison
Cytogenetic Survey.

Presentation Type: Poster
Session: P, Comparative Genomics and Transcriptomics
Location: Exhibit Hall/Omni Hotel
Date: Monday, June 26th, 2017
Time: 5:30 PM This poster will be presented at 5:30 pm. The Poster Session runs from 5:30 pm to 7:00 pm. Posters with odd poster numbers are presented at 5:30 pm, and posters with even poster numbers are presented at 6:15 pm.
Number: PGT003
Abstract ID:266
Candidate for Awards:Genetics Section Poster Award