Create your own conference schedule! Click here for full instructions

Abstract Detail

Symbioses: Plant, Animal, and Microbe Interactions

Neupane, Surendra [1], Nepal, Madhav P. [1], Ma, Qin [2], Mathew, Febina [2], Varenhorst, Adam [3].

Quantifying induced susceptibility effects in soybean-aphid interaction.

It is essesntial to understand how soybean respond to its econiomincally important pests (soybean cyst nematode [SCN=Heterodera glycines Ichinohe], and the soybean aphid [=Aphis glycines Matsumura])to develop resistant soybean (Glycine max L.) cultivars,. Previous studies have indicated that interactions between the two pests can compromise the soybean health. In this research, we are characterizing candidate genes in soybean that are differentially regulated upon infection by the soybean aphid and SCN. We conducted a greenhouse experiment using randomized complete block design with 3 blocks and 12 treatments. The treatments were combinations of infesting resistant and susceptible soybean with inducers, response, no inducers and no response populations of aphids. We infested soybean aphid- resistant (Rag1; LD12-15813Ra) and susceptible soybean (LD12-15838R) with inducer populations that were either 50 biotype-1 or biotype-2 soybean aphids on the first trifoliate stage. After 24 hours, each plant received a response population of five soybean aphids on the second trifoliate. We observed the induced susceptibility effects on soybean-aphid interactions. In susceptible cultivars both treatments and blocks were significant (F values: 10.950, 4.497 at p<0.05) whereas, in resistant cultivars only treatment was significant (F value: 7.470 at p<0.05). After 1, 5 and 11 days of aphid-infestation, we collected leaf tissues through flash freezing in liquid nitrogen and stored at -80 degree Celcius. RNA work is under progress to identify the differentially expressed genes and potential candidate genes conferring resistance to the two pests. Data obtained from this research will have implication in developing broad resistance of soybean to SCN and soybean aphid.

Log in to add this item to your schedule

Related Links:
Nepal Lab @SDSU

1 - South Dakota State University , Biology and Microbiology , McFadden Biostress Laboratory, Brookings , SD, 57007
2 - South Dakota State University, Plant Science, McFadden Biostress Laboratory, Brookings, SD, 57007
3 - South Dakota State University, Plant Science, Berg Agricultural Hall, Brookings, SD, 57007

Induced susceptibility effect 
Soybean-Aphid interaction
gene expression
RNA seq
Soybean cyst nematode (SCN).

Presentation Type: Oral Paper
Session: 24, Symbioses: Plant, Animal, and Microbe Interactions
Location: Fort Worth Ballroom 5/Omni Hotel
Date: Tuesday, June 27th, 2017
Time: 10:15 AM
Number: 24002
Abstract ID:265
Candidate for Awards:None

Copyright © 2000-2017, Botanical Society of America. All rights reserved