| Abstract Detail
Classical Genetics Finch, Kristen [1], Cronn, Richard [2]. Source identification of western Oregon Douglas-fir using wood molecular abundance data and SNPs. We investigated if wood metabolite profiles from Direct Analysis in Real Time (Time of Flight) Mass Spectrometry (DART-TOFMS) and genetic markers could be used to determine the geographic origin of Douglas-fir wood cores originating from two regions in western Oregon, USA. Three annual ring mass spectra were obtained from 188 adult Douglas-fir trees, and 141 trees from the same populations were genotyped for 16,000 SNPs using an Affymetrix array. We separately analyzed both wood chemical profiles and ten principal components based on genotypes using random forest models to determine whether samples could be classified to geographic origin via wood molecules or genetics alone, and we combined datasets to quantify the geographic classification accuracy obtained with a combination of wood metabolite data and genotypic data. Specific wood molecules and genotypes that contributed to the geographic discrimination were identified. Douglas-fir mass spectra could be differentiated into two geographic classes with an accuracy of 76%, and principal components of genotypes could distinguish Douglas-fir trees to locations with an accuracy of 80%. Combining molecular data and genetic data improved upon genetic classification accuracy by only 1%. We identified thirty-two molecules and 50 SNPs as key for classifying western Oregon Douglas-fir wood cores and individual trees to geographic origin. DART-TOFMS is capable of detecting minute but regionally-informative differences in wood molecules over a small geographic scale, and these differences make it possible to predict the geographic origin of Douglas-fir wood with moderate accuracy using wood chemistry alone. Genetic information provides greater classification accuracy and spatial resolution, but requires more resources for development. Studies involving DART-TOFMS, alone and in combination with other technologies, will be relevant for identifying the geographic origin of illegally harvested wood.
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1 - Oregon State University, Botany and Plant Pathology, 2082 Cordley Hall, 2701 SW Campus Way, Corvallis, OR, 97331, United States
2 - USDA Forest Service, 3200 SW Jefferson Way, Corvallis, OR, 97330, USA
Keywords:
Douglas-fir
Pseudotsuga
Metabolites
provenance
wood identification
genomics
SNPs
random forests.
Presentation Type: Oral Paper
Session: 4, Classical Genetics & Molecular Ecology
Location: Fort Worth Ballroom 8/Omni Hotel
Date: Monday, June 26th, 2017
Time: 9:00 AM
Number: 4004
Abstract ID:245
Candidate for Awards:Margaret Menzel Award |
