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Abstract Detail

Physiology & Ecophysiology

WU, HONGHONG [1], Shabala, Lana [2], Shabala, Sergey [2], Giraldo, Juan Pablo [3].

Cerium oxide nanoparticles improve Arabidopsis salinity stress tolerance by enabling leaf mesophyll K+ retention.

Salinity is a critical factor limiting agricultural production worldwide. Efficient mesophyll K+ retention is one of the most important sub-traits of salinity tolerance. Here, we report that negatively charged poly (acrylic acid) coated cerium oxide nanoparticles (PNC) protect plant performance under salinity stress. Leaves infiltrated with PNC (PNC-leaves) have significantly higher carbon assimilation rates, stomatal conductance, quantum yield, maximum efficiency of photosystem II and chlorophyll content compared with controls without nanoparticles after being exposed to 100 mM NaCl for 3 days. Confocal imaging of reactive oxygen species (ROS) generation in planta and whole leaf histochemical staining demonstrated that PNC-leaves exhibit less ROS levels in leaf mesophyll cells than controls. Electrophysiological experiments showed that PNC-leaves have about three times lower NaCl-induced K+ efflux from mesophyll cells than the buffer control. This was confirmed by confocal imaging with K+ dye APG-2 showing that PNC infiltrated leaves have higher cytosolic K+ in leaf mesophyll cells than buffer controls. Furthermore, relative expression of K+ uptake plasma membrane transporter HAK5 gene was significantly upregulated in PNC-leaves under salinity stress, compared with no significant change on K+ efflux plasma membrane channel GORK gene. Overall, our results demonstrate that scavenging of excess ROS by PNC imparts plants with enhanced ability to maintain K+ in the cytosol of leaf mesophyll cells and therefore better salinity tolerance.

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1 - UC Riverside, Botany and Plant Sciences, 3401 Watkins Drive, Batchelor Hall 1133, Riverside, CA, 92521, USA
2 - University of Tasmania, Australia, School of Land and Food, College Road, Life Science Building, Room 445 , Hobart, TAS, 7005, Australia
3 - UC Riverside, Botany and Plant Sciences, 3401 Watkins Drive, Batchelor Hall 1125, Riverside, CA, 92521, USA

cerium oxide nanoparticles
ion channels/transporters
ion fluxes
mesophyll K+ retention
salt stress.

Presentation Type: Oral Paper
Session: 23, Physiology & Ecophysiology
Location: Fort Worth Ballroom 6/Omni Hotel
Date: Tuesday, June 27th, 2017
Time: 11:00 AM
Number: 23009
Abstract ID:152
Candidate for Awards:None

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