Abstract Detail

Comparative Genomics/Transcriptomics

RUSSELL, SCOTT D [1], Anderson, Sarah [2], Jones, Daniel [3], Chesnut, Joshua [3], Johnson, Cameron [2], Khanday, Imtiyaz [2], Sundaresan, V [2], Gou, Xiaoping [4].

Transcriptional Profiles of In Vivo Fertilized Rice Gametes (Oryza sativa L).

Microdissected mature, newly-fertilized egg cells and early zygotes were isolated to assay transcriptional changes during maternal to zygotic transition (MZT) in the early zygote. In animal models, expression is essentially exclusively material for >10 mitotic cycles, but MZT is poorly described for plants. Rice (Oryza sativa) was chosen as a model system because gametes and zygotes can be directly isolated and because of the amount of genomic information available. Expression profiles were examined prior to and during early post-fertilization stages at 1.5 hours to 9 hours after pollination. Egg-cell/zygote expressed genes displayed that some genes showed signal intensity increases in the newly-fertilized egg cell, whereas many showed decreases. Paternal transcripts were found in zygotes at all stages examined but at lower abundance than maternal transcripts. This is expected as the volume of the egg cells exceed that of the sperm cells by up to a 1:1000 difference and suggest that sperm transcripts may not be detected early stages of the fertilized zygote. Newly-expressed transcripts were detected in transition from unfertilized to fertilized egg cells while other transcripts became undetectable. Initial expression relative to zygote activation presumably mainly concerns changes in expression relating to early zygotic changes in expression relating to early stages in the zygote in polarization, the completion of the cell cycle and initiation of S-phase and ultimately zygotic division.

1 - University Of Oklahoma, Department Of Microbiology And Plant Biology, 770 Van Vleet Oval, Norman, OK, 73019-6155, USA
2 - University of California, Department of Plant Biology, Davis, CA, 95616, USA
3 - University Of Oklahoma, Department of Microbiology & Plant Biology, Norman, OK, 73019, USA
4 - Lanzhou University, School of Life Sciences, Lanzhou, Ganzu, China

Keywords:
rice
zygote
Transcriptome
embryogenesis.

Presentation Type: Oral Paper
Session: 10, Comparative Genomics and Transcriptomics
Location: Fort Worth Ballroom 5/Omni Hotel
Date: Monday, June 26th, 2017
Time: 1:45 PM
Number: 10002
Abstract ID:137
Candidate for Awards:None