Abstract Detail

Systematics

Jeon, Ji-Hyeon [1], Maki, Masayuki [2], Kim, Seung-Chul [1].

Inferring phylogenetic relationships among recently diverged East Asian species of roses (Rosa section Synstylae; Rosaceae).

Of ten sections of genus Rosa, section Synstylae includes ca. 25 species mostly occurring in East Asia. Section Synstylae and its closely related section Chinenses are well circumscribed primarily based on exerted styles in a column and include most of the wild ancestors of modern cultivated roses. In particular, section Synstylae is one of the most recently diverged sections in genus Rosa. Due to their short lineage, neither conventional molecular phylogenetic markers of nuclear nor chloroplast ones can resolve phylogenetic relationships among species within sect. Synstylae, or delimit species boundaries. Even chloroplast genomes of those shared identical sequences except for only few SNPs or indels. Albeit nuclear markers showed more diverse sequence than chloroplast ones, sequence divergences were not sufficient enough to resolve phylogenetic relationships among the species with good nodal supports. Though recently developed several NGS-based genomic data are extremely valuable to reconstruct phylogenetic relationships of such recently diverged and closely related species, the highly conserved single copy genes could also be quite useful for resolving close non-model species relationships. As Cabrera et al. (2009) developed Rosaceae Conserved Orthologous Set (RosCOS) markers from single copy Arabidopsis genes, we tested those RosCOS markers on Rosa sect. Synstylae species especially focusing on Rosa multiflora and its close relatives. Of the markers on the RosCOS database (Cabrera et al., 2009), we selected a total of six markers of distinct genomic regions to resolve their phylogenetic relationships and recover their relationship supports in Rosa sect. Synstylae. With maximum likelihood analysis (ML), every single one of six markers showed high variation rates both among and within species, delimiting each species boundary with relatively high nodal support value in phylogenetic tree. While each of those showed slightly different genealogy, supermatrix concatenating sequence data of six markers suggested well-resolved species phylogeny. Those markers also distinguished the putative hybrid accessions in neighbor-joining (NJ) phylogenetic network, suggesting intensive genetic or genomic introgression among those species. Our results suggest that RosCOS markers are highly variable and phylogenetically informative compared to conventional molecular markers to resolve complex relationships complex relationships of recently diverged and highly reticulated lineages of Rosa section Synstylae with high support values. Moreover, robust phylogenetic frameworks based on RosCOS markers would be helpful to reveal complex evolutionary and biogeographic history of one of phylogenetically challenging genera in the rose family.

1 - Sungkyunkwan University, Department of Biological Sciences, 2066, Seobu-ro, Suwon, Gyeonggi, 16419, Republic of Korea
2 - TOHOKU UNIV, Botanical Gardens, Kawauchi 12-2, Aoba, SENDAI, MIYAGI, N/A, 980-0862, Japan

Keywords:
Rosa
section Synstylae
phylogeny
RosCOS.

Presentation Type: Oral Paper
Session: 14, Systematics I: Basal Dicots, Monocots & Rosids
Location: Sundance 1/Omni Hotel
Date: Monday, June 26th, 2017
Time: 5:00 PM
Number: 14015
Abstract ID:86
Candidate for Awards:None